Gabriela M. Mimendi-Aguilar, Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico
Martha R. Hernández-Preciado, Departamento de Disciplinas Filosófico Metodológicas e Instrumentales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico
Jesús A. Juárez-Ozuna, Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico
Ingrid P. Dávalos-Rodríguez, División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, Mexico
Erick Sierra-Díaz, División de Epidemiología, UMAE Hospital de Especialidades, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social. Guadalajara, Jalisco, Mexico
Maria C. Morán-Moguel, epartamento de Disciplinas Filosófico Metodológicas e Instrumentales, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico
Introduction: Prostate cancer (PCa) is the most common cancer diagnosed in men. The KISS1 gene encodes the KISS1 protein, also called metastin or kisspeptin, which inhibits metastasis without altering malignant transformation of the tumor. The rs12998 variant is a missense mutation related to the risk of developing metastasis. The allelic and genotypic frequencies in the Mexican population and whether it is associated with metastasis in patients with PCa are unknown. Objective: To determine the genotypic and allelic frequencies of the rs12998 variant in the general population and patients with PCa. Method: Genomic DNA from prostate tissue of 40 individuals with PCa (with or without metastasis) and 90 individuals from the general population was analyzed. The variant was identified by PCR-RFLPs with the restriction enzyme NlaIV. Results: In the general population, the genotype frequencies of rs12998 were: C/C = 0.91; C/T = 0.09 and T/T = 0; while the C/C genotype was present in all patients with prostate cancer with and without metastasis. Conclusions: The KISS1 rs12998 variant is in Hardy-Weinberg equilibrium in the general Mexican population. This variant is not a possible predictive genetic marker of metastasis in Mexican patients with prostate cancer.
Keywords: Prostate cancer. KISS1 gene. Metastasis. Prognostic marker.
